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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry
doi: 10.1016/j.xpro.2025.103684
Figure Lengend Snippet: Surface and intracellular antibody cocktails for antibody titrations
Article Snippet:
Techniques:
Journal: STAR Protocols
Article Title: Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry
doi: 10.1016/j.xpro.2025.103684
Figure Lengend Snippet: Summary table of markers expressed for each cell type in mouse lungs, spleen and peritoneal lavage
Article Snippet:
Techniques:
Journal: STAR Protocols
Article Title: Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry
doi: 10.1016/j.xpro.2025.103684
Figure Lengend Snippet: UMAP plots of mouse cells analyzed by mass cytometry UMAP is a dimensionality reduction technique, which allows summarizing n dimensions data into two dimensions. These graphs show the UMAP plots for cells isolated from thawed (A) and fresh (B) spleen of mice infected with Echinococcus multilocularis , cells isolated from fresh spleen of uninfected mouse (C), thawed (D) and fresh (E) peritoneal cells of mice infected with E. multilocularis , and cells isolated from fresh lungs of uninfected mouse (F). Gates show B cells (green), T CD4 + cells (orange), T CD8 + cells (blue), monocytes/macrophages/dendritic cells (red), eosinophils (pink), neutrophils and precursors (black) and undetermined populations (gray). Thawed cells (especially myeloid cells) had modified expression of some markers, compared to fresh cells (A vs. B, and D vs. E, respectively). This modified slightly their UMAP distribution and can interfere with analysis and interpretation process.
Article Snippet:
Techniques: Mass Cytometry, Isolation, Infection, Modification, Expressing
Journal: STAR Protocols
Article Title: Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry
doi: 10.1016/j.xpro.2025.103684
Figure Lengend Snippet:
Article Snippet:
Techniques: Purification, Blocking Assay, Recombinant, Sterility, Staining, Red Blood Cell Lysis, Antibody Labeling, Software, Membrane, Transferring, Spectrophotometry